Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites
نویسندگان
چکیده
منابع مشابه
Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique
Mapping sites within the genome that are hypersensitive to digestion with DNaseI is an important method for identifying DNA elements that regulate transcription. The standard approach to locating these DNaseI-hypersensitive sites (DHSs) has been to use Southern blotting techniques, although we, and others, have recently published alternative methods using a range of technologies including high-...
متن کاملGenome-wide identification of DNaseI hypersensitive sites using active chromatin sequence libraries.
Comprehensive identification of sequences that regulate transcription is one of the major goals of genome biology. Focal alteration in chromatin structure in vivo, detectable through hypersensitivity to DNaseI and other nucleases, is the sine qua non of a diverse cast of transcriptional regulatory elements including enhancers, promoters, insulators, and locus control regions. We developed an ap...
متن کاملAnalysis of Mice Lacking DNaseI Hypersensitive Sites at the 5′ End of the IgH Locus
The 5' end of the IgH locus contains a cluster of DNaseI hypersensitive sites, one of which (HS1) was shown to be pro-B cell specific and to contain binding sites for the transcription factors PU.1, E2A, and Pax5. These data as well as the location of the hypersensitive sites at the 5' border of the IgH locus suggested a possible regulatory function for these elements with respect to the IgH lo...
متن کاملNative genomic blotting: high-resolution mapping of DNase I-hypersensitive sites and protein-DNA interactions.
DNase I-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. We have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic DNA on native polyacrylamide gels prior to electroblotting and hybridization. This improved method has been used to scan th...
متن کاملPredicting gene regulatory elements in silico on a genomic scale.
We performed a systematic analysis of gene upstream regions in the yeast genome for occurrences of regular expression-type patterns with the goal of identifying potential regulatory elements. To achieve this goal, we have developed a new sequence pattern discovery algorithm that searches exhaustively for a priori unknown regular expression-type patterns that are over-represented in a given set ...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2006
ISSN: 1088-9051
DOI: 10.1101/gr.5373606